![]() Aquaporins (AQPs), also called water channels, form a 13-member (AQP0–12) transmembrane protein family. Given that water accounts for 80% of the brain volume, glioma often leads to brain edema and hernia, further increasing the risk of mortality. Gliomas have a very high recurrence rate therefore, the average life expectancy in patients with glioma is less than 15 months. Surgical resection of tumor tissue and chemotherapy cannot achieve the ideal therapeutic effect because of glioma cells’ rapid proliferation and high invasiveness. Glioma is the most common primary intracranial tumor and one of the most diverse and complex cancers in humans. Therefore, AQP8 may be a potential therapeutic target in gliomas. Our results preliminary suggest that AQP8 overexpression alters the ROS/PTEN/AKT signaling pathway, promoting the proliferation, migration, and invasion of gliomas. In the animal experiments, the AQP8 overexpression group had higher tumor volume and weight, whereas the AQP8 knockdown group had lower tumor volume and weight compared with those parameters measured in the control group. Overexpression of AQP8 resulted in an increased number of cell clones and cell proliferation, enhanced cell invasion and migration, decreased apoptosis and phosphatase and tensin homolog (PTEN) expression, and increased phosphorylated serine/threonine protein kinase (p-AKT) expression and ROS level, whereas the AQP8 knockdown groups showed opposite results. A nude mouse tumor model was also established. The effects of AQP8 on the proliferation and growth of glioma and its mechanism via the intracellular reactive oxygen species (ROS) level were observed using cell clone, transwell, flow cytometry, Hoechst, western blotting, immunofluorescence, and real-time quantitative polymerase chain reaction assays. The dCas9-SAM and CRISPR/Cas9 techniques were used to construct viruses with overexpressed and knocked down AQP8, respectively, and infect A172 and U251 cell lines. This study aimed to investigate the mechanism and role of abnormal AQP8 expression in glioma development. However, the mechanism by which AQP8 promotes the proliferation and growth of glioma remains unclear. Expression of AQP8 is higher in human glioma tissues than in normal brain tissues and is positively correlated with the pathological grade of glioma, suggesting that this protein is also involved in the proliferation and growth of glioma. The aquaporin (AQP) family of proteins has been implicated in the proliferation and growth of gliomas. ![]()
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